negative stain meaning in English
负染剂
阴性染剂
Examples
- Negative staining technique
负染色法 - The virus could multifly on the mdck cells . and induce regular cytopathic effect ( cpe ) . observed by negative staining electron microscope , the virus could be seen with coroniform spike . in the ultrathin sections , the viruses were found to multiply and induce inclusion body forming in the cytoplasm
本研究从某动物园两只死亡大熊猫的肝、脾等脏器分离病毒，经mdck细胞适应培养，获得一株病毒(命名为dxmv ) ，在该细胞上可形成有规律的病变。 - In this thesis , the fist cyanophage isolated in china was investigated with regard to the molecular biological characters . techniques for the large harvest and the electronic microscopy negative staining of the cyanophage were established . the genome library was constructed
论文对我国淡水中分离得到的一株蓝藻病毒(噬藻体)的某些分子生物学特性进行了研究，建立了其大量增殖与电镜染色的技术，构建了基因组文库，并对部分序列进行了测定与和分析比较。 - The his - tagged peacl - gfp purified from the supernatants could polymerize into green fluorescent filamentous structures with diameter , length and shape being identical to that of muscle f - actins , which could be labeled by tritc - phalloidin ( a specific agent for staining actin microfilaments ) , and were identified as having a 9 nm diameter by negative staining , corresponding with that of the muscle f - actins ( 7 - 10 nm ) . under polymerization conditions , his - tagged peacl - gfp polymerized with kinetics similar to those of skeleton muscle actin , that is , an obvious lag nucleation period at the beginning of polymerization and an s - like typical polymerization curve could be obtained . the critical concentration is 0 . 75 umol / l near to that of chicken muscle actin ( 0 . 56 umol / l ) under the same condition
荧光标记结合荧光显微观察表明：从可溶性上清中纯化的his - taggedpeac1 - gfp聚合形成的微丝不仅可以直接在荧光显微镜下观察，也可被微丝的特异标记物鬼笔环肽所标记，而且其直径、长度以及形态上与已知的聚合肌动蛋白荧光丝一致；电镜负染的结果进一步证实其直径为9nm ，与传统微丝直径相当( 7 ? 10nm ) ；聚合曲线有明显的停滞期，为典型的s型聚合曲线，聚合临界浓度为0 . 75 mol l ，这一结果与已有报道相似。